Name
In vitro and in vivo analysis using mono-reassortants of bovine rotavirus A constructed by a reverse genetics
Presenter
Tohru Suzuki, National Institute of Animal Health
Co-Author(s)
Tohru Suzuki 1, Yuta Kanai 2, Shohei Minami 2, Tomohiro Kotaki 2, Takeshi Kobayashi 2
1 Sapporo Research Station, National Institute of Animal Health, NARO, 2 Department of Virology, Research Institute for Microbial Diseases, Osaka University
Abstract Category
Pathogenesis and Immunity
Abstract
Bovine rotavirus A is a major causative pathogen of diarrhea in dairy and Japanese beef calves, which has led to severe economic losses in Japan every year. The pathogen has 11 double-stranded RNA segments encoding six viral proteins (VP1-4, VP6 and VP7) and five/six nonstructural proteins (NSP1-6). The two capsid proteins, VP7 and VP4, of which induce neutralizing antibodies and represent G and P genotypes respectively. The dual genotyping system targeted on VP7 and VP4 has been used to understand epidemiological characterization of RVAs at the global level. Among bovine RVAs, three G genotypes (G6, G8, G10) and three P genotypes (P[1], P[5], P[11]) are the most common. Beside them, the bovine RVAs with G21P[29] and G24P[33] genotypes have been isolated from asymptomatic cows in Japan.
Reverse genetics is one of useful approaches to clarify infectious mechanism of RVA. This study firstly has been established recombinant bovine RVA with G6P[1] genotype. Subsequently, multiple mono-reassortant RVAs have been constructed by replacing VP7 and VP4 of this recombinant RVA with those from bovine RVAs with other G genotypes and P genotypes. By comparing and analyzing biological activity in vitro and vivo model using these mono-reassortants, and antigenicity via cross-reactivity between these and antisera obtained from calves infected with bovine RVAs of three different genotypes, our study aimed to understand antigenicity and pathogenicity of bovine RVAs. In this symposium, we will present the results obtained via the present analysis.
Reverse genetics is one of useful approaches to clarify infectious mechanism of RVA. This study firstly has been established recombinant bovine RVA with G6P[1] genotype. Subsequently, multiple mono-reassortant RVAs have been constructed by replacing VP7 and VP4 of this recombinant RVA with those from bovine RVAs with other G genotypes and P genotypes. By comparing and analyzing biological activity in vitro and vivo model using these mono-reassortants, and antigenicity via cross-reactivity between these and antisera obtained from calves infected with bovine RVAs of three different genotypes, our study aimed to understand antigenicity and pathogenicity of bovine RVAs. In this symposium, we will present the results obtained via the present analysis.