Name
Evaluation of sensitivity and specificity of New Immunochromatograpghy (IC) kit for the rapid detection of rotavirus associated acute gastroenteritis in Dhaka, Bangladesh
Presenter
Shuvra Kanti Dey, Jahangirnagar University
Co-Author(s)
Shuvra Kanti Dey, Evana Zaman Khan, Sazzad Khan, Abdullah Ibna Masud, Jannatin Naim, Fariha Bushra Khan, Nadim Sharif
Department of Microbiology, Jahangirnagar University, Dhaka-1342, Bangladesh
Abstract Category
Epidemiology, Evolution, and Diversity
Abstract
Background: Acute gastroenteritis (AGE) is a leading cause of illness and death in children globally, with diarrhea playing a prominent role. Besides different molecular techniques, immunochromatography kit can detect rotavirus within a very short time. Our research intended to evaluate the kits used for rotavirus by comparing the detection results with the reference method RT-PCR.
Methods: Between June 2023 and January 2024, 110 fecal specimens were collected from children in Tangail, Bangladesh, who had diarrhea. The IP-Rota/Noro/Adeno kit (Immuno-Probe, Japan) was used to detect rotavirus. The method is a lateral flow immunochromatographic assay, where viral antigen forms a complex with specific monoclonal antibody-conjugated gold colloid in the pad and moves onto the membrane by capillary action within the test plate. The sensitivity and specificity of the immunochromatography (IC) kit were determined using the gold standard RT-PCR and PCR techniques.
Results: Among 110 stool specimens 76, 11 and 7 specimens were detected as rotaviurus, norovirus and adenovirus positive by IC kit. All specimens were subjected to further analysis by RT-PCR method. In compare with RT-PCR method, the sensitivity and specificity of IC kit for the detection of rotavirus was 96% and 88% and for norovirus was 100% and 99% and for adevirus was 100% and 98.1% respectively.
Conclusion: IC kit will be easy and useful assay for the rapid detection of rotavirus. Finally, it must be suggested the the IC kit might be used as an alternative method for the diagnosis of rotavirus, especially in developing countries like Bangladesh.
Methods: Between June 2023 and January 2024, 110 fecal specimens were collected from children in Tangail, Bangladesh, who had diarrhea. The IP-Rota/Noro/Adeno kit (Immuno-Probe, Japan) was used to detect rotavirus. The method is a lateral flow immunochromatographic assay, where viral antigen forms a complex with specific monoclonal antibody-conjugated gold colloid in the pad and moves onto the membrane by capillary action within the test plate. The sensitivity and specificity of the immunochromatography (IC) kit were determined using the gold standard RT-PCR and PCR techniques.
Results: Among 110 stool specimens 76, 11 and 7 specimens were detected as rotaviurus, norovirus and adenovirus positive by IC kit. All specimens were subjected to further analysis by RT-PCR method. In compare with RT-PCR method, the sensitivity and specificity of IC kit for the detection of rotavirus was 96% and 88% and for norovirus was 100% and 99% and for adevirus was 100% and 98.1% respectively.
Conclusion: IC kit will be easy and useful assay for the rapid detection of rotavirus. Finally, it must be suggested the the IC kit might be used as an alternative method for the diagnosis of rotavirus, especially in developing countries like Bangladesh.