Name
Rotavirus and Clostridium perfringens from a municipal eutrophic lake of Kashmir Himalaya- Detection and Toxinotypes
Presenter
IRFAN AHMAD, Faculty of Fisheries. SKUAST-Kashmir, India
Co-Author(s)
Irfan Ahmad1*, Anjum Afshan2 & Aasifa Jan3
1 . Div. of Genetics & Biotechnology, FOFy, SKUAST.K, Rangil, Kashmir, India-06
kirmani9@yahoo.co.in
* Presenting author
2. Department of Anaesthesia & Critical Care, King Fahads Specialist Hospital, Dammam, KSA, Formerly
Department of Anaesthesia, GB Panth Children Hospital, Srinagar, Kashmir, India.
3 Department of Humanities. JK-School Education Deptt. Jammu & Kashmir. India.
Abstract Category
Epidemiology, Evolution, and Diversity
Abstract
Background
Both Rotavirus and Clostridium perfringens were detected in water samples collected from an urban eutrophic lake. Incidence of rota viral disease is 45% under four years of age. C. perfringens was detected in all water samples from all sites and presence of rotavirus was detected from only one site.
Methods: Sample collection. Extraction of RNA and DNA. PCR, Typing and sequencing for homology.
The sewage fed area had direct inflow of human excreta. The virus was detected throughout the season from Jan to Dec. The electrophoretic patterns of the positive samples showed small and long e-types. The physiochemical parameters of the site revealed mean water temp (0C) 18.1, min 5.4 (Jan), max 28.7 (July). pH mean 8.1, min 7.6 (Aug), max 8.8 (Jan) and DO mg/L mean 7.3, min 5.2 (Oct), max 9.2 (Dec). The genome persistence of the virus, eutrophication and pesticidal leaching pose a severe threat to the toxicity of endemic snow trouts inhabiting these aquatic habitats.
Toxinotyping of C. perfringens from water revealed the presence of type A strain (carrying only α-toxin gene) . Phylogenetic analysis of α-toxin gene and protein of the C. perfringens type A isolates were suggestive of some common strains circulating in India, esp. in the NE regions. Thus water accounts to be reflected as an imminent source of C. perfringens type A infection to human populaces via the food chain and might pose a serious public health concern as the lake is used as a biggest vegetable bowl of city.
Both Rotavirus and Clostridium perfringens were detected in water samples collected from an urban eutrophic lake. Incidence of rota viral disease is 45% under four years of age. C. perfringens was detected in all water samples from all sites and presence of rotavirus was detected from only one site.
Methods: Sample collection. Extraction of RNA and DNA. PCR, Typing and sequencing for homology.
The sewage fed area had direct inflow of human excreta. The virus was detected throughout the season from Jan to Dec. The electrophoretic patterns of the positive samples showed small and long e-types. The physiochemical parameters of the site revealed mean water temp (0C) 18.1, min 5.4 (Jan), max 28.7 (July). pH mean 8.1, min 7.6 (Aug), max 8.8 (Jan) and DO mg/L mean 7.3, min 5.2 (Oct), max 9.2 (Dec). The genome persistence of the virus, eutrophication and pesticidal leaching pose a severe threat to the toxicity of endemic snow trouts inhabiting these aquatic habitats.
Toxinotyping of C. perfringens from water revealed the presence of type A strain (carrying only α-toxin gene) . Phylogenetic analysis of α-toxin gene and protein of the C. perfringens type A isolates were suggestive of some common strains circulating in India, esp. in the NE regions. Thus water accounts to be reflected as an imminent source of C. perfringens type A infection to human populaces via the food chain and might pose a serious public health concern as the lake is used as a biggest vegetable bowl of city.