Name
Infectious bursal disease virus (IBDV) VP3 contains a predicted C-terminal intrinsically disordered region (IDR) that modulates the physical properties of virus factories (VFs)
Presenter
Andrew Brodrick, University of Maryland College Park
Co-Author(s)
Andrew James Brodrick1, Andrew James Broadbent1 1
Department of Animal and Avian Sciences, University of Maryland, 8127 Regents Drive, College Park, MD, 20742, USA.
Abstract Category
Virus Replication: Entry, Exit and Everything in Between
Abstract
The virus factories (VFs) of infectious bursal disease virus (IBDV) form through liquid-liquid phase separation (LLPS). A major component of the IBDV VF is the nonstructural protein VP3. Here, we predicted the full-length structure of the VP3 monomer and homodimer and employed molecular dynamics simulations to characterize their behavior. We identified the 36 amino acid carboxy(C)-terminus as a highly dynamic intrinsically disordered region (IDR). We then compared the cytoplasmic puncta that were made in the presence of the wild type (wt) VP3 with those made with a VP3 that lacked the C-terminus (VP3∆C). Using live-cell imaging with fluorescent reporter tagged proteins, we found that VP3∆C puncta were significantly less numerous (p